The FT4/T3 ratio showed a significant decreasing trend when the 1st and 4th days of the walk were compared (Fig. 4). The activation of the HPA axis represents a physiological response to the energetic, metabolic, vascular, neurophysiological, and psychological requirements of exercise . Therefore, the decrease trend in cortisol response found was not as expected. Exercise above this threshold increases the plasma cortisol concentration linearly with exercise intensity . Cortisol is therefore an exercise-responsive hormone that shows a significant increase after high intensity and short duration exercise or submaximal intensity exercise with a longer duration . Analysis of the weight of the 28 participants, specifically the change in daily weight (weight before-weight after walking), showed a loss of − 1.8% of the total weight on the 1st day; − 0.52% on the 2nd day; − 0.8% on the 3rd day; and a paradoxical gain of + 1% on the final day (Table 2). Studying these hormonal changes, particularly during walking, the most popular physical activity, may help better understand the hormonal homeostasis during this type of activity. Physical activity is a strong stimulus for the endocrine system, and the hormonal response to exercise is regulated by several factors, including intensity, individual level of training, duration, and type of exercise (endurance vs. resistance) . These findings highlight an adaptive hormonal mechanism in response to stress that is repeated daily, as shown by cortisol and thyroid function in our study. If you’re lifting weights or doing intense training, walking is the perfect recovery-day activity. Kraemer et al. reported that, when the number of repetitions during exercise was kept constant, the intensity determined the degree of acute post-exercise increase in serum testosterone concentrations. 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